Izazinzulu zenze okokuqala i-hydrogel etofwayo edibanisa ngaphambili iimolekyuli ze-bioactive ezithi ngqo ngokusebenzisa inoveli crosslinkers. I-hydrogel echazwe inamandla anamandla okusetyenziswa kubunjineli beethishu
Tissue ubunjineli kuphuhliso lwezicubu kunye nezinto ezithatha indawo ye-organ - ezintathu-dimensional cellular constructs - zineepropati ezifanayo nezicubu zendalo. Ukwenza i-engineering ijolise ekubuyiseleni, ekulondolozeni okanye ekwandiseni imisebenzi yethishu ngokusetyenziswa kwezi scaffolds zebhayoloji. Ukwenziwa ihydrogel Iipolymers ziye zanconywa njengabagqatswa abathembisayo ukubonelela ngezikafula ezinomatshini ngenxa yokwakheka kwazo okwahlukileyo kunye nokufana kolwakhiwo kunye ne-matrix yendalo yangaphandle. I-Hydrogels ilinganisa imeko yezicubu kunye ne-crosslinkers kwi-hydrogels inceda imathiriyeli ukugcina ubume bayo nokuba ifunxa amanzi amaninzi. Nangona kunjalo, ii-hydrogels ezikhoyo ngoku zingasebenzi ngokwebhayoloji kwaye ke azikwazi kusebenza ngokuzimeleyo ukuqhuba umsebenzi ofanelekileyo webhayoloji. Bafuna ukongezwa kwee-biomolecules ezihambelanayo (umzekelo wezinto zokukhula, i-adhesive ligands) ezenza zibe yinxalenye ebalulekileyo yee-hydrogels.
Kuphononongo olupapashwe ngoJuni 11 ngo Uphuhliso lwezesayensi, izazinzulu ziye zavelisa i-modular injectable hydrogel entsha esebenzisa i-crosslinker ebizwa ngokuba yi-PdBT - i-biodegradable compound - i-crosslinking ye-polymer ye-hydrogel ukudala i-swollen, i-bioactive hydrogel. I-PdBT idibanisa iimolekyuli ze-bioactive ngokuziqinisa kwiikhemikhali ze-crosslinkers kwi-hydrogel. I-biomolecules ethile inokuxutywa ngokulula kunye ne-PdBT kwiqondo lokushisa lokushisa kwaye ngokwenza njalo ii-molecule ze-bioactive zibe yinxalenye edibeneyo ye-hydrogel. Inkqubo enjalo, ephuhliswe okokuqala, inamandla okuzibophelela kwii-biomolecules ze-tissue-specific kubushushu begumbi ukuze zisebenze ngaphandle kokufuna naliphi na i-injection yesibini okanye inkqubo kamva.
I-biomolecules eyongeziweyo ihlala igxininiswe kwi-hydrogel kwaye inokubonakaliswa ngokuthe ngqo kwi-tissue ekujoliswe kuyo. Oku kuthintela ukusasazwa kwindawo engaphandle kwendawo ekujoliswe kuyo ukuthintela iziphumo ezingafunekiyo ezifana nokungasebenzi okanye ukukhula kwezicubu ezingafunekiyo. Iimvavanyo zenziwe kwithambo kunye ne-cartilage usebenzisa i-PdBT monomers ethile ngokongeza ukusebenza ngokubandakanya i-cartilage ehambelana ne-hydrophobic N-cadherin peptide kunye ne-hydrophilic bone morphogenetic protein peptide, kunye ne-cartilage-derived glycosaminoglycan, i-chondroitin sulfate. Lo mxube we-hydrogel unokujoliswa ngokuthe ngqo kwi-tissue ekujoliswe kuyo. Ii-biomolecules ezifakwe kwi-hydrogel zidibana neeseli zomzimba ze-mesenchymal stem ze-host host kwaye "zilungele" ukuze zongezwe kwindawo ekujoliswe kuyo 'kwimbewu' okanye ziqalise ukukhula okutsha. Emva kokuba izicubu ezitsha zikhula, i-hydrogel iyancipha kwaye iyanyamalala.
I-hydrogel entsha echazwe kuphononongo lwangoku inokulungiswa kwiqondo lokushisa kwegumbi ukuze isetyenziswe ngokukhawuleza kwaye inokwenziwa ngokwezifiso ngokufanelekileyo kwizicubu ezahlukeneyo. Inkqubo yokulungiselela ngokuthe tye inqanda ukuthotywa kwe-thermal ye-biomolecules eye yaba ngumcimbi kunye ne-hydrogels yangaphambili njengoko oku kuchaphazela umsebenzi wabo we-biological. I-bioactive hydrogels inokunceda ukuvuselela ithambo, i-cartilage, ulusu kunye nezinye izihlunu. Obu buchwephesha benoveli obusebenzisa i-hydrogel etofwayo ye-bioactive eneempawu ezithandekayo inamandla amakhulu okusetyenziswa kubunjineli beethishu.
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{Unokufunda iphepha lokuqala lophando ngokunqakraza ikhonkco le-DOI elinikwe ngezantsi kuluhlu lwemithombo ecatshulweyo (s)}
Umthombo (s)
UGuo JL et al. 2019. Imodyuli, izicubu ezithe ngqo, kunye ne-biodegradable hydrogel cross-linkers kubunjineli beethishu. Ukuqhubela phambili kwezeNzululwazi. 5 (6). https://doi.org/10.1126/sciadv.aaw7396
