I-Spinal Cord Injury (SCI): Ukusebenzisa i-Bio-active scaffolds ukubuyisela umsebenzi

Ukuzihlanganisa nanostructures yenziwe kusetyenziswa supramolecular Iipholima equlethe i-peptide amphiphiles (PAs) equlethe ulandelelwano olusebenzayo lwe-bio ibonise iziphumo ezinkulu kwimodeli yemouse ye-SCI kwaye ibambe isithembiso esikhulu, ebantwini, ngokusebenzayo. impatho yale meko ibuthathaka echaphazela kakhulu umgangatho wobomi kunye nempilo yengqondo yabachaphazelekayo abantu, kunye namalungu abo entsapho kwaye ngumthwalo onzima kwinkqubo yokunakekelwa kwezempilo kunye nentlalontle. 

A umqolo ukulimala, okuhlala kubangelwa ukubetha ngokukhawuleza okanye ukunqunyulwa komqolo, kukhokelela ekulahlekelweni okusisigxina kwamandla, ukuvakalelwa kunye nomsebenzi ongaphantsi kwendawo yokulimala. Nangona kungekho nonyango olusekelwe ngokufanelekileyo lokulimala okunjalo, amanqaku amaninzi ophando ashicilelwe ukuze aqonde i-molecular pathology yokulimala komgogodla kwaye eze neengcebiso zokuvuselela izicubu ezichaphazelekayo, ngaloo ndlela kukhuthazwa ukubuyiswa komsebenzi kwaye emva koko kuvumele abantu ukuba bakhokele. ubomi obunemveliso ngakumbi kunye nokuzimela. Ukuqhubela phambili kwisayensi kunye nethekhnoloji yokuqonda iindlela zemolekyuli eziphantsi kokulimala komnqonqo kunye neendlela ezicebisayo zonyango, ukongeza kwisixhobo sokubuyisela kwimeko yesiqhelo kunye nezixhobo ezincedisayo, ziya kuhamba indlela ende ekubuyiselweni kwabantu kuloo monzakalo unzima kwaye ubancede ukuba bakhokele ngakumbi. ubomi obunentsingiselo. 

Kwinqaku lamva nje elipapashwe kwiSayensi nge-11 kaNovemba ka-2021, u-Alvarez kunye noogxa bakhe bavavanya iipolymers ze-supramolecular ezine-peptide amphiphiles (PAs), kwimodeli yempuku yokwenzakala komntu okhubazekileyo (SCI)^1. Ezi PA ziqulethe izibonakaliso ezimbini ezicacileyo, eyokuqala ivula i-receptor ye-transmembrane i-β1-integrin kunye neyesibini isebenze i-fibroblast factor growth factor 2 receptor. I-Peptide amphiphiles (i-PAs) ziimolekyuli ezincinci eziqulethe amacandelo e-hydrophobic adityaniswe ngokudibeneyo kumtya wee-amino acids (peptides). Ulandelelwano lwe-peptide lunokwenziwa ukwenza i-β-sheets, ngelixa iintsalela ezikude nomsila zihlawuliswa ukukhuthaza ukunyibilika kwaye zinokuqulatha ulandelelwano lwe-bioactive. Emva kokunyibilika emanzini, ezi PAs zenza i-β-sheet formation kunye nokuwa kwe-hydrophobic yemisila ye-aliphatic kwaye yenze ukuhlanganiswa kweeamolekyuli zibe yi-supramolecular one-dimensional nanostructures (umzekelo, i-high-aspect-ratio cylindrical okanye i-ribbonlike nanofibres). INdibano idla ngokunyanzeliswa kukugxininiswa okungafaniyo, i-pH kunye nokuqaliswa kwee-divalent cations^2,3. Ezi nanostructures zibaluleke kakhulu kwimisebenzi ye-biomedical ngenxa yokukwazi ukubonisa ukuxinana okuphezulu kweempawu zebhayoloji kumphezulu wazo wokujolisa okanye ukuvuselela iindlela. 

Ngokudala iinguqu kulandelelwano lwepeptide kwi-non-signal, i-non-bioactive domain, i-intense supramolecular motion ngaphakathi kwe-nanofibres yabonwa, ngaloo ndlela iphucula ukubuyiswa kwe-SCI. Uguquko olunamandla amakhulu, alubanga nesiphumo sokukhula kwakhona kwe-axon kunye ne-myelination, kodwa lukhokelele ekwakhiweni kwemithambo yegazi (i-revascularization), kunye nokusinda kwe-motor neuron. 

Ezi polymers ze-supramolecular ezine-peptide amphiphiles (PAs) ngaloo ndlela zinesithembiso esikhulu ekuncedeni abantu ukuba babuyele kwii-SCIs, ezinokuthi zibe neziphumo ezibi kakhulu kubomi bezigulana, emzimbeni nasemoyeni. Ngaphaya koko, ezi nanostructures zokuzihlanganisa, zenziwe ngeepolima ze-supramolecular ezine-peptide amphiphiles (PAs), zinokusetyenziselwa usetyenziso olwahlukeneyo lwebhayoloji njenge. iziyobisi ukuhanjiswa, ukuvuselelwa kwamathambo kunye nokunciphisa ukulahleka kwegazi ngexesha lokuphuma kwangaphakathi. 

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Ucaphulo 

1. Álvarez Z., okqhubekayo 2021. I-Bioactive scaffolds ene-supramolecular motion ephuculweyo ikhuthaza ukuchacha kwi-spinal cord. Inzululwazi. Ipapashwe 11 Nov 2021. Vol 374, Issue 6569. iphepha 848-856. DOI: https://doi.org/10.1126/science.abh3602 

2. Hartgerink, JD; uBeniash, E.; I-Stupp, i-SI Peptide-Amphiphile Nanofibers: I-Scaffold eNxulutywayo yokuLungiselela iZixhobo zokuZihlanganisa. Iproc. Natl. Akhad. Sci. USA 2002, 99, 5133-5138, DOI: https://doi.org/10.1073/pnas.072699999 

3. Pashuck, ET; Kuyi, H.; Stupp, SI Tuning Supramolecular Rigidity of Peptide Fibers through the Molecular Structure. J. Am. Ikhem. Soc. 2010, 132, 6041-6046, DOI: https://doi.org/10.1021/ja908560n 

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