Izazinzulu zenze iqonga le-3D le-bioprinting elihlanganisa ukusebenza umntu izicubu ze-neural. Iiseli zeprogenitor kwizicubu eziprintiweyo zikhula zenze iisekethe ze-neural kwaye zenze unxibelelwano olusebenzayo kunye nezinye ii-neuron ngaloo ndlela zilingisa indalo. ngengqondo izicubu. Le yinkqubela phambili ebalulekileyo kubunjineli bezicubu ze-neural kunye neteknoloji ye-3D bioprinting. Izicubu ezinjalo ze-bioprinted neural zingasetyenziselwa ukwenza imodeli umntu izifo (ezifana ne-Alzheimer's, i-Parkinson's njl.) ezibangelwa ngenxa yokuphazamiseka kwenethiwekhi ye-neural. Naluphi na uphando lwezifo zengqondo lufuna ukuqonda indlela umntu iinethiwekhi ze-neural ziyasebenza.
3D bioprinting yinkqubo eyongezelelweyo apho i-biomaterial efanelekileyo yendalo okanye yokwenziwa (i-bioink) ixutywe kunye neeseli eziphilayo kwaye ziprintwe, umaleko-ngamaleko, kwizakhiwo zendalo ezifana ne-three-dimensional structures. Iiseli zikhula kwi-bioink kwaye izakhiwo zikhula ukulinganisa izicubu zendalo okanye ilungu. Le teknoloji ifumene izicelo kwi Ukuhlaziywa ngokutsha iyeza le-bioprinting yeeseli, izicubu kunye namalungu kunye nophando njengemodeli yokufunda umntu umzimba in vitro, ngakumbi umntu inkqubo ye-nervous.
Ukufunda kwe umntu inkqubo ye-nervous ijongene nemida ngenxa yokungafumaneki kweesampuli eziphambili. Iimodeli zezilwanyana ziluncedo kodwa zinengxaki yokwahlukana kweentlobo ezithile kungoko kunyanzelekile in vitro iimodeli ze umntu inkqubo yemithambo-luvo ukuphanda indlela umntu uthungelwano lwe-neural lusebenza ekufumaneni unyango lwezifo ezibalelwa kukonakaliswa kothungelwano lwe-neural.
umntu Izicubu ze-neural ziye zashicilelwa kwi-3D kwixesha elidlulileyo kusetyenziswa iiseli ze-stem kodwa ezi ziswele ukwakhiwa kwenethiwekhi ye-neural. Ithishu eprintiweyo ayizange ibonise ukuba yenze unxibelelwano phakathi kweeseli ngenxa yezizathu ezininzi. Ezi ntsilelo zoyiswe ngoku.
Kuphononongo lwakutshanje, baphandi yakhetha i-fibrin hydrogel (ebandakanya i-fibrinogen kunye ne-thrombin) njengesiseko se-bioink kwaye icwangciswe ukuprinta isakhiwo esineleya apho iiseli ze-progenitor zinokukhula kwaye zenze ii-synapses ngaphakathi kunye nangaphaya kweeleya, kodwa zatshintsha indlela iileya ezipakishwe ngayo ngexesha loshicilelo. Endaweni yendlela yemveli yokupakisha iileya ngokuthe nkqo, bakhethe ukuprinta iileya ecaleni komnye ngokuthe tye. Kuyabonakala ukuba oku kwenza umahluko. Iqonga labo le-3D le-bioprinting lifunyenwe ukuba lidibanise ukusebenza umntu izicubu ze-neural. Uphuculo ngaphezu kwamanye amaqonga akhoyo, i umntu Izicubu ze-neural eziprintwe leli qonga zenze uthungelwano lwe-neural kunye nonxibelelwano olusebenzayo kunye nezinye ii-neurons kunye neeseli zeglial ngaphakathi naphakathi kweeleya. Le yimeko yokuqala enjalo kwaye linyathelo elibalulekileyo eliya phambili kubunjineli bezicubu ze-neural. Ukudityaniswa kwelabhoratri yemithambo-luvo elinganisa ubuchopho ekusebenzeni kuvakala kumnandi. Le nkqubela ngokuqinisekileyo iya kunceda abaphandi kwimodeli umntu izifo zengqondo ezibangelwa ngenxa yokuphazamiseka kwenethiwekhi ye-neural ukuqonda ngcono indlela yokufumana unyango olunokwenzeka.
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Iingxelo:
- Cadena M., okqhubekayo 2020. I-3D Bioprinting yeeNeural Tissues. IiMathiriyeli zoNyango lwezeMpilo oluPhezulu uMqulu 10, ukukhutshwa kwe-15 2001600. I-DOI: https://doi.org/10.1002/adhm.202001600
- Yan Y., okqhubekayo 2024. 3D bioprinting of umntu izicubu ze-neural ezinonxibelelwano olusebenzayo. Cell Stem Cell Technology| Umqulu 31, ushicilelo 2, P260-274.E7, ngoFebruwari 01, 2024. DOI: https://doi.org/10.1016/j.stem.2023.12.009
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