I-fungus i-Penicillium roqueforti isetyenziselwa ukuveliswa kwetshizi ene-blue-veined. Indlela echanekileyo esemva kombala owodwa ozuba-luhlaza wetshizi ayizange iqondwe kakuhle. KwiYunivesithi yaseNottingham abaphandi baye batyhila indlela i-classic blue-green veining eyenziwa ngayo. Bafumanisa a I-canonical DHN-melanin biosynthetic pathway in P. roqueforti eyathi ngokuthe ngcembe yenze ibala elizuba. 'Ngokuvalela' indlela kwiindawo ezithile, iqela lenze uluhlu olubanzi lweentlobo zokungunda ngemibala emitsha. Iintlobo zokungunda ezintsha zinokusetyenziselwa ukwenza 'isonka samasi esibhlowu' ngemibala eyahlukileyo ukusuka komhlophe ukuya kumthubi-luhlaza ukuya kobomvu-mdaka-pinki kunye nokukhanya kunye noluhlaza okwesibhakabhaka.
Ngundo I-penicillium roqueforti isetyenziswa kwihlabathi jikelele kwimveliso yesonka samasi eblue-veined ezifana neStilton, Roquefort kunye neGorgonzola. Umngundo udlala indima ebalulekileyo kwincasa kunye nophuhliso lwendalo ngomsebenzi wayo we-enzymatic. Uphawu, imbonakalo ye-blue-vein of cheese ibangelwa yi-pigmentation ye-spores eyenziwe ngokungaqhelekanga kwimingxuma yetshizi. Umbala oyingqayizivele ohlaza okwesibhakabhaka weshizi ubaluleke kakhulu kwintengiso.
Nangona kunjalo, isiseko sofuzo/imolekyuli ye-spore pigmentation ye P. roqueforti ayiqondwa ngokucacileyo.
Ukusebenzisa indibaniselwano ye-bioinformatics kunye ibhayiloji yemolekyuli ubuchule, iqela lophando leYunivesithi yaseNottingham liphande ukuba kwakheka njani umbala oluhlaza-luhlaza wesonka samasi. Ubukho kunye nendima ye-DHN-melanin biosynthesis pathway in I-Aspergillus fumigatus sele ichaziwe kungoko isalathiso sobukho bendlela enye kwi-P. roqueforti nayo. Le ndlela inemizila emithandathu esebenza ngokulandelelana kwe-enzyme eyaziwa ngokudibanisa i-DHN-melanin. Iqela lophando lichonge ngempumelelo i-canonical DHN-melanin biosynthetic pathway kwi-P. roqueforti. Iseti efanayo ye zendalo zaye zachongwa zaza zalandelelana ukusuka kwiisampuli ze-P. roqueforti ezisetyenziselwa umsebenzi wokulinga.
ICanonical DHN-imelanin I-biosynthetic pathway ngokuthe ngcembe yenze i-pigments eluhlaza okwesibhakabhaka, iqala kumbala omhlophe, othi ngokuthe ngcembe ube tyheli-luhlaza, obomvu-brown-pinki, omdaka obumnyama, oluhlaza okwesibhakabhaka, kwaye ekugqibeleni luhlaza okwesibhakabhaka obumnyama.
Iqela ke lasebenzisa iindlela ezifanelekileyo 'ukuvala' indlela kwiindawo ezithile kwaye livelise uluhlu olubanzi lweentlobo ngemibala emitsha.
Ngaphaya koko, baphanda ngeentlobo ezintsha zokungcamla kwaye bafumanisa ukuba incasa yeentlobo ezitsha yayifana kakhulu nohlobo oluluhlaza oluvela kuzo. Nangona kunjalo, ulingo lwencasa luveze ukuba umbono wencasa nawo waphenjelelwa ngumbala.
Iziphumo zolu phononongo zingasetyenziswa kwi ushizi ukuveliswa kwemibala eyahlukeneyo kunye neencasa.
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Imbekiselo:
- Cleere, MM, Novodvorska, M., Geib, E. okqhubekayo. Imibala emitsha emidala kwifungus ye-blue-cheese Penicillium roqueforti. npj Sci Ukutya 8, 3 (2024). https://doi.org/10.1038/s41538-023-00244-9
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