Uvavanyo kwiiseli zeempuku lubonisa indlela entsha ekhomba kwiinzuzo ezinokubakho zekhokhonathi yokulawula Isifo se-Alzheimer
Isifo se-Alzheimer yinkqubela ngengqondo Ukuphazamiseka okuchaphazela abantu abazizigidi ezingama-50 kwihlabathi liphela. Akukho nyango lufunyenweyo okwangoku IAlzheimer; ezinye iindlela zonyango ezikhoyo zinokukhulula kuphela iimpawu ezinxulumene nesifo. IAlzheimer Isifo sibonakala ngokuqina kweplaque enganyibilikiyo (yeamyloid beta proteins) phakathi kwemithambo yemithambo-luvo. ngengqondo. Oku kukhokelela ekugqithiseni ukuhanjiswa kweempembelelo kwii-neurons kwaye kubangela iimpawu ze IAlzheimer isifo – ikakhulu ukuwohloka kwenkumbulo. I-Amyloid beta 40 kunye ne-Amyloid beta 42 iiprotheyini zifumaneka kakhulu plates. Amyloid beta protein zixhomekeke ekubonakalisweni kweprotein ye-amyloid precursor (APP). Uphando luseke ukubaluleka kweprotein ye-amyloid precursor kwi IAlzheimer isifo. Ukuncipha okungaphelelanga komsebenzi we-APP kubonwa njengonyango lwe-Alzheimer's, nangona eyona ndlela ichaza uqokelelo lwe-amyloid beta protein ayikaqondwa ngokupheleleyo.
Izifundo ezininzi kwixesha elidlulileyo zibonise ukuba intombi amafutha lwawo inokuba nefuthe kwiindlela ezininzi ezithi ke zibe negalelo kwinkqubela phambili ye IAlzheimer isifo. I-oyile yeKhokhonathi ibandakanya ubukhulu becala i-acids efunxayo ephakathi kwitsheyina elinamafutha etyoliswe lula sisibindi. Ezi asidi ezinamafutha zinokuguqulwa zibe yi-ketones - zithathwa njengomthombo wamandla we-neurons. Ioli yeCoconut ibonakaliswe ukuba ineempembelelo ezichasene ne-oxidant ekukhuseleni i-neurons. Ezi mpawu zenza ioyile yekhokhonathi ibe ngamanqatha okutya awodwa.
Kuphononongo lwamva nje olupapashwe kwi ingqondo Research, abaphandi baye baphanda imiphumo enokubakho yeoyile yekakhukhunathi ekubonakalisweni kweprotheni ebalulekileyo ye-amyloid precursor (APP) ejongene nokwakheka kwe-amyloid plaque. Abaphandi baphonononga ukubonakaliswa kweprotheyini ye-amyloid ye-precursor kunye nokukhuselwa kwe-amyloid peptides kwi-mammalian cell line Neuro 2A (okanye i-N2a) iiseli ebonisa uhlobo lwe-APP. Lo mgca weseli ye-neural usetyenziswa rhoqo ukufunda ukuhlukana kwe-neuronal, ukukhula kwe-axonal kunye neendlela zokubonisa. Kuphononongo lwangoku, iiseli ze-N2a zifumana unyango nge-0-5 yeepesenti ze-coconut yeoli kwaye oku kwakhokelela ekunciphiseni i-protein ye-amyloid ye-precursor ibonakaliso kwiiseli kunye nokunciphisa i-secretion ye-amyloid peptides 40 kunye ne-42. Ukongezelela ioli ye-coconut nayo yakhuthaza i-N2a iiseli Ukwahlula kubonisa ukuba ioli yekakhukhunathi inefuthe elikhuselayo ekuphuhlisweni kweeseli ze-neuronal.
Iziphumo zibonise ukuba i-ADP-Ribosylation Factor 1 (ARF1) - a protein kubalulekile kwindlela eyimfihlo - inokuba negalelo kwimiphumo yeoli yekhokhonathi kuzo zombini inkcazo ye-APP kunye ne-amyloid peptides secretion. Kwakucacile ukuba ioyile yekhokhonathi iphumelele oku ngokusebenzisana okunokwenzeka kunye ne-ARF1. I-ARF1 yaziwa ngokuba noxanduva lokuhlela nokuthutha iiproteni zedyasi kwiseli. Eli lixesha lokuqala umbutho phakathi kwe-ARF1 kunye ne-amyloid precursor protein (APP) processing iboniswa. Lo mbutho ulawulwa ngonyango lweoli yekakhukhunathi. Ukunkqonkqoza ngaphandle kwe-ARF1 kuncitshiswe ukukhuselwa kwe-amyloid peptides ukuseka indima yeprotheni ye-ARF1 kulawulo lwe-APP.
Uphononongo luchaza indima engazange ichazwe ngaphambili yeoli yekakhukhunathi ekunciphiseni i-amyloid precursor protein (APP) inkcazo kunye nemfihlo ye-peptides ye-amyloid, umphumo ophunyeziweyo ngenxa yokulawulwa kwe-ARF1. Ngaloo ndlela, i-ARF1 inoxanduva lokuthutha i-APP ngaphakathi kwe-neurons ngelixa ioli yekakhukhunathi ichaphazela umsebenzi kunye nokubonakaliswa kwe-APP. Uphononongo lunika iinkcukacha ngembono entsha yokurhweba nge-intracellular ye-amyloid precursor protein kwaye oku kubalulekile ukuqonda isifo sika-Alzheimer.
Olu phononongo lucebisa ukuba ukusebenzisa ioyile yekhokhonathi ekutyeni kwasebutsheni bobomi bomntu, ngakumbi kubantu abaxhomekeke kwimfuza. IAlzheimer isifo ngenxa yembali yosapho, inokulibazisa okanye ide imise ukuqala kwesi sifo. Izifundo zangoku kunye nezexesha elidlulileyo ziqinisekisa uphando olongezelelweyo kunye nezilingo zeklinikhi zabantu ukuvavanya umthamo kunye nokhuseleko lweoyile yekhokhonathi. Ioyile yeKhokhonathi ayibizi, ifumaneka lula kwaye inokufakwa ngokulula kwisidlo sezigulane ezisemngciphekweni.
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{Unokufunda iphepha lokuqala lophando ngokunqakraza ikhonkco le-DOI elinikwe ngezantsi kuluhlu lwemithombo ecatshulweyo (s)}
Umthombo (s)
I-Bansal A et al 2019. Ioyile yeCoconut iyanciphisa ukubonakaliswa kweprotheni ye-amyloid ye-precursor (APP) kunye ne-secretion ye-amyloid peptides ngo-inhibition ye-ADP-ribosylation factor 1 (ARF1). Uphando ngengqondo. https://doi.org/10.1016/j.brainres.2018.10.001
